Naoko watanabe. Naoko Watanabe (voice actress) 2019-10-23

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Naoko Watanabe's research works

naoko watanabe

Among 80 isolates of E. This study investigates the potential of dairy waste management practices to alter the amount of monensin available for release into the environment. Environmental release of veterinary pharmaceuticals has been of regulatory concern for more than a decade. Analysis of wastewater and groundwater from two dairy farms in California consistently concluded that monensin is most present in lagoon water and groundwater downgradient of lagoons. Results and discussion In the G-treatments, urease activity decreased with higher sulfonamide concentrations; no effect was observed when no glucose was added W-treatments. No pathogenic bacteria were detected in groundwater from 200 small supply wells in the extended survey.

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Naoko Watanabe's research works

naoko watanabe

While a dose–response relationship was observed for urease activity after 168 h, a similar inhibition was measured after 380 h at all sulfonamide concentrations. Watanabe was a corporate associate in a large international law firm, representing clients in a wide range of corporate transactions, including public and private mergers and acquisitions, joint ventures and corporate finance. The ubiquity of microbial matter poses unique technical challenges in addition to economic constraints when sampling wells in such environments. . We use cookies on our website. This study investigates the potential of monensin to move from dairy operations into the surrounding ground water.

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Naoko Watanabe (voice actress)

naoko watanabe

On the other hand, constructed wetlands have also been shown to remove such bacteria Ibekwe et al. The most frequent antibiotic detections were associated with lagoons, hospital pens, and calf hutches. Of these 10, sulfadimethoxine, sulfamethazine, and lincomycin were found in shallow groundwater directly downgradient from the lagoons. A variety of antibiotics were applied at both farms leading to antibiotics excretion of several hundred grams per farm per day. Theoretical maximum concentration estimated from the actual dosing rate and the theoretical excretion rate assuming no attenuation was one order of magnitude greater than observed concentrations, suggesting significant attenuation in the manure collection and storage system. Sulfadimethoxine and sulfamethazine were detected in shallow groundwater near field flood irrigation gates, but at highly attenuated levels. The data suggest the possibility of monensin transport into shallow 2-5 m alluvial ground water from dairy management units, including manure storage lagoons and freestalls occupied by heifers, lactating cows, and dry cows.

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Naoko Watanabe's research works

naoko watanabe

This result is in agreement with those obtained in microbial groundwater sampling protocol for fecal-rich environments, where the authors observed that the levels of enterococci in the monitoredwater samples were initially very highand later stabilized. She is a native Japanese speaker and has worked in Japan at leading automotive companies, Bridgestone Corporation and Honda Motor Co. Using two dairy farms in California as study sites, we twice collected samples along the environmental pathway-from flush lanes, lagoon waters, and shallow ground water beneath the dairies and beneath its associated manured fields. The assessment shows that extended purging can successfully control for equipment cross-contamination, but also controls for significant contamination of the well-head, within the well casing and within the immediate aquifer vicinity of the well-screen. Monensin was detected in all of the flush lane and lagoon water samples. Among a subset of E.

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Naoko Watanabe's research works

naoko watanabe

Screen is used in wastewater treatment in order to eliminate large particles that may cause damage to pumps and blocking of downstream 86. Further research is needed addressing both long-term effects and lower sulfonamide concentrations under dynamic boundary conditions. Concentrations of both generic E. The soil microbial community was affected by sulfonamides even at a relatively low concentration, although this soil receives regular input of manure that contains several antibiotics. Purpose Up to 90% of antibiotics that are fed to livestock are excreted unaltered or as metabolites and thus are present in manure. Prior to joining the Firm, Ms.

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Naoko Watanabe (actress)

naoko watanabe

Soil 700 g was amended with either mineral water only W-treatments , liquid manure M-treatments , or with glucose solution G-treatments. Monensin was also detected, at levels ranging from 0. Monensin is a feed additive antibiotic that is prevalent throughout the dairy industry and is excreted in dairy waste. Pharmaceuticals used in animal feeding operations have been detected in various environmental settings. Wendorff 89 suggested the utilizing of a wire screen and grit chamber with a screen orifice size of 9.

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Naoko Watanabe (voice actress)

naoko watanabe

In a prior study with steers beef cattle fed with monensin at 330 mg · day 1 per cow, 40 to 50% of the dosed monensin was excreted in the manure 19 , which is considerably higher than in our study. By weight, monensin can be the most significant antibiotic used in a dairy farm. The results indicate that monensin attenuation is not improved by increased solid-liquid separation prior to storage in lagoons, as monensin is rapidly desorbed after dilution with water. By application of manure as fertilizer, veterinary antibiotics can reach soil and groundwater. In this paper, we evaluate a groundwater sampling protocol that relies on extended purging with a portable submersible stainless steel pump and Teflon ® tubing as an alternative to equipment sterilization. The aim of this study is to determine the effect of three commonly used and simultaneously applied sulfonamide antibiotics on both function and structural diversity of soil microorganisms.

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Naoko Watanabe's research works

naoko watanabe

Monensin is also shown to be easily degraded in lagoon microcosms receiving aeration, but is relatively stable and available for leaching under typical anaerobic lagoon conditions. Yet, despite decades of use, antibiotic occurrence appeared constrained to within farm boundaries. The protocol allows for collecting a large number of samples quickly, relatively inexpensively, and under field conditions with limited access to capacity for sterilizing equipment. Strong inhibition of urease by manure M-treatments was observed even without the addition of sulfonamides. No other study was found in the literature that reported monensin excretion rates for dairy cows, and we were not able to calculate such rates from the reported monensin concentra- tions in lagoon samples from dairy farms 20. Since the lagoons represent a direct source of monensin to groundwater, the effect of waste management, by mechanical screen separation and lagoon aeration, on aqueous monensin concentration was investigated through construction of lagoon microcosms.

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Naoko Watanabe

naoko watanabe

In particular, sulfamethazine, a sulfonamide antibiotic, was detected at a relatively high concentration along with monensin, a feed additive, and hormonal drugs. Subsurface media soil, nat- ural sediment act as natural filters for microorganisms through several processes like straining and physical filtration or bacterial adsorption and adhesion to solid media Murphy and Ginn, 2000. This response might also occur to microbial communities in antibioticcontaminated river ecosystems but may be modulated by the difference in proportion of bacteria, algae, and fungi among different benthic substrata cobbles, sediment, leaves Artigas et al. Antibiotics or other chemicals and contaminants present in environmental matrices, contribute to this further by offering selective pressure, thus allowing for their survival and expansion Martinez, 2009. Antibiotics were sporadically detected in field surface samples on fields with manure applications, but not in underlying sandy soils. Monensin concentrations were determined using solid-phase extraction and liquid chromatography-tandem mass spectrometry with positive electrospray ionization. Conclusions Sulfonamides clearly affected both the function and structural diversity of the soil microbial community over at least 16 days.

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Naoko Watanabe's research works

naoko watanabe

Fecal contamination of surface water, river water, wetlands, and even drinking water have been implicated in the spread of such resistance Baquero et al. In manure lagoons, 10 compounds were detected including tetracyclines and trimethoprim. Although legis- lation assume that these processes are sufficient enough to retain bacte- ria in the top meter of the vadose zone, the presence of allochthonous microorganisms e. There is a growing concern about the impact on terrestrial and aquatic organisms and the development of antibiotic-resistant strains of microorganisms. Generic Escherichia coli was isolated from surface water and groundwater samples from two dairies in Northern California and tested for susceptibility to antibiotics.

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